Chemical Isotope Labeling LC-MS for Human Blood Metabolome Analysis.
| Author | |
|---|---|
| Abstract |
:
Blood is a widely used biofluid in discovery metabolomic research to search for clinical metabolite biomarkers of diseases. Analyzing the entire human blood metabolome is a major analytical challenge, as blood, after being processed into serum or plasma, contains thousands of metabolites with diverse chemical and physical properties as well as a wide range of concentrations. We describe an enabling method based on high-performance chemical isotope labeling (CIL) liquid chromatography-mass spectrometry (LC-MS) for in-depth quantification of the metabolomic differences in comparative blood samples with high accuracy and precision. |
| Year of Publication |
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0
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| Journal |
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Methods in molecular biology (Clifton, N.J.)
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| Volume |
:
1730
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| Number of Pages |
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213-225
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| Date Published |
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2018
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| ISSN Number |
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1064-3745
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| URL |
:
https://dx.doi.org/10.1007/978-1-4939-7592-1_14
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| DOI |
:
10.1007/978-1-4939-7592-1_14
|
| Short Title |
:
Methods Mol Biol
|
| Download citation |
